摘要
针对绿藻,采用超声波提取,以荧光激发-发射波长差216nm,建立了同步荧光法测定绿藻叶绿素a含量的新方法,其线性范围为0.02～1.25 mg/L,检出限为1.6ug/L,加标回收率在97.0%～104%之间。与分光光度法测定叶绿素a的对比试验结果表明,两者无显著性差异,但该方法具有快速、灵敏,其他常见色素不干扰测定的优点,能够满足供水中绿藻叶绿素a含量快速检测的需要。
A method for the determination of chlorophyll a in chlorella by ultra-sound extraction and synchronous fluorescence spectrometry with constant—wavelength interval of 216 nm is developed.The linear range is 0.02-1.25 mg/L with the detection limit of 1.6ug/L.The recovery is between 97.0% and 104%.By comparison with the visible spectrophotography,they have not significant differences.The method is quick and sensitive.Other common pigments do not interfere.It can meet the demands of testing green algae.
出处
《中国人口·资源与环境》
CSSCI
北大核心
2011年第S1期192-194,共3页
China Population,Resources and Environment
基金
国家水体污染控制与治理科技重大专项"引黄供水地区水质风险评估技术研究与应用"(编号:2008ZX07422-001)
关键词
同步荧光
超声波提取
叶绿素A
绿藻
synchronous fluorescence spectrometry
ultra sound extraction
chlorophyll a
green algae
