固体食品中苯并[α]芘提取及检测方法的改进

Improvement of determination and extraction of 3,4-benzo(α) pyrene in solid food

因传统的提取方法存在耗时长、有机溶剂使用量较大的缺点,且荧光法测定操作繁琐,本法希望通过改进提取方法及检测方法,达到既消除固体食品基质中干扰又为检测工作提高效率的目的。本实验先萃取固体样品中油样,继而从油样中提取苯并[α]芘,经高效液相色谱法测定后,由样品中脂肪含量换算得到苯并[α]芘在样品中含量。实验结果显示,平均加标回收率为98.3%,相对标准偏差为2.6%,检出限为0.1μg/kg。该法可较大程度地清除固体样品杂质干扰,节省有机溶剂使用量,同时提高提取效率。

The traditional extraction method is a time consuming method,and it has to be used with a great quantity of organic solvent. Furthermore,the paper chromatography part before the fluorimetric spectrometry is a very complicated job. The objective of this research is to increase efficiency and remove the most impurity interference in solid food by improve both extraction and determination method. 3,4-benzo(α) pyrene was extracted from oil samples which were obtained from solid food first,and the content of 3,4-benzo(α) pyrene can be calculated from the fat content of samples. The recovery of this method was 98.3%,RSD was 2.6%(n=5) ,and the detection limit of 3,4-benzo(α) pyrene was 0.1 μg/kg. The result shows that this method can remove the most impurity interference in solid food sample,and it will save a lot of organic solvents without reducing the extraction efficiency.