固相萃取-液质联用同时检测豆制品中4种红黄色系工业染料

Simultaneous determination of 4 industrial dyes in soybean products by solid phase extraction-liquid chromatography-tandem mass spectrometry

目的以WAX固相萃取柱净化处理,建立豆制品中违禁添加色素碱性橙Ⅱ、碱性嫩黄O、酸性橙Ⅱ和罗丹明B的前处理方法。方法利用电喷雾离子源(ESI),碱性橙、碱性嫩黄和罗丹明B采用正离子模式,酸性橙采用负离子模式,建立正负离子切换的多反应监测(MRM)的检测方法。结果碱性橙Ⅱ、碱性嫩黄O的检出限为1.0g/kg,酸性橙Ⅱ和罗丹明B的检出限为0.5g/kg,方法回收率为84.295.1%,相对标准偏差(RSD)小于4.5%。结论建立的液质联用方法具有快速、灵敏、准确等优点,可用于豆制品中上述4种非法添加的红黄色系工业染料的同时测定及确证。

Objective To develop a pre-treatment method for the 4 illegal added dyes of ChrysoidineⅡ,Auramine O,OrangeⅡ and Rhodamine B in soybean products purified with WAX solid phase extraction column.Methods Positive ESI mode was used for ChrysoidineⅡ,Auramine O and Rhodamine B while negative ESI mode for OrangeⅡ,the positive-negative switch MRM detection method was developed.Results The LOD for ChrysoidineⅡand Auramine O was 1.0 g/kg,while it was 0.5 g/kg for OrangeⅡand Rhodamine B,the recovery rate for this method was from 84.2 to 95.1%,the RSD values were less than 4.5%.Conclusion The obtained liquid chromatography-tandem mass spectrometric method is fast,sensitive and accurate,and it can be used for the analysis and confirmation of the 4 illegally added industrial dyes in soybean products.