LC-MS/MS法测定保健食品中左卡尼汀的含量

Determination of levocarnitine in health care food by LC-MS/MS

目的建立保健品中左卡尼汀的LC-MS/MS的测定方法。方法待测样品经超声提取、稀释后,分别采用CAPCELLPAKCR色谱柱和PCHILIC色谱柱进行分离,最后用LC-MS/MS进行检测。结果使用CAPCELLPAKCR法检测左卡尼汀能够得到更高的灵敏度,线性范围为0～200ng/mL,相关系数为r=0.9995。结论该方法灵敏度高、准确、重现性好,可以用于一些保健食品中左卡尼汀的含量检测。

Objective To establish a method for the determination of levocarnitine in health care food by LC-MS/MS.Methods After an ultrasonic extraction and dilution,the samples were separated by CAPCELL PAK CR and PC HILIC column,respectively.Finally the target compound was detected by LC-MS/MS.Results Compared with the PC HILIC column,using CAPCELL PAK CR column could get a higher sensitivity result.The method showed a good linearity over the range of 0～200.0 ng/mL for levocarnitine with r=0.9995.Conclusion This method has a good sensitivity,accuracy and reproducibility and it is suitable for the detection of levocarnitine in health care food.