反相高压液相色谱法测定蜂胶保健食品中白杨素、高良姜素的含量

Determination of chrysin and galangin in propolis health care foods by reversed-phase high pressure liquid chromatography

目的建立用反相高压液相色谱测定保健食品中白杨素、高良姜素的方法。方法蜂胶样品中白杨素、高良姜素经甲醇水浴回流提取、C18反相柱分离,在波长268nm处检测。结果此方法得到的白杨素回收率为95.5%～100.8%,RSD为1.1%～2.0%,高良姜素回收率为95.2%～100.5%,RSD为1.6%～2.8%。结论此方法简便快速,准确可靠,可用于对一些蜂胶保健食品中白杨素、高良姜素含量的测定。

Objective To establish a method for the detection of chrysin and galangin in propolis health care foods by reversed-phase high pressure liquid chromatography(RP-HPLC).Methods Chrysin and galangin in samples were reflux extracted with methanol in water bath,separated by C 18 reversed phase column,and detected at wavelength of 268 nm.Results The recovery of chrysin was 95.6%～100.8% with the relative standard deviations of 1.1%～2.0%,and the recovery of galangin was 95.2%～100.5% with the relative standard deviations of 1.6%～2.8%.Conclusion The method is simple,rapid,accurate and reliable,which could be used for the detection of chrysin and galangin in propolis health care foods.