基于体外消化模型的食品中邻苯二甲酸酯生物有效性的测定

Determination of bioaccessibility of phthalate acid esters in food based on an in vitro digestion model

目的建立固相萃取-高压液相色谱法检测体外消化模型消化液及固相残渣中邻苯二甲酸酯(PAEs)的方法.方法食品经体外消化模型处理后离心分为固液两相,分别经溶剂提取、固相萃取柱净化后采用HPLC检测两相中的PAEs含量,通过计算得到生物有效性值.结果该方法在0.1~20 mg/L范围内线性关系良好,相关系数均大于0.998,方法的回收率在70.38%~96.49%之间,相对标准偏差均低于7%.结论该方法操作简单,灵敏度和重现性好,抗干扰能力强,可用于食品中PAEs生物有效性的测定,同时为进一步进行PAEs暴露评估和健康风险评估奠定基础.

Objective An solid-phase extraction-high pressure liuid chromatograpy (SPE-HPLC) method was developed for determination of four kinds of phthalate acid esters(PAEs) in liquid phase and solid phase of digestive juice base on an in vitro digestion model. Methods The food sample was treated via the in vitro di-gestion model, and the digestive juices were centrifuged to separate supernatant and solid. After extracted using n-hexane and purified by SPE (Florisil column), PAEs in the liquid phase and solid phase were determined by HPLC, respectively, and then the bioaccessibility were calculated. Results The method showed a good linear-ity over the range of 0.1~20.0 mg/L for PAEs with r=0.998. The recoveries were in range of 70.38%~96.49%, and the relative standard deviations(RSDs) were lower than 7%. Conclusion The proposed method with strong anti-interference capacity is convenient, sensitive and accurate, and it is suitable for the determination of bioaccessibility of PAEs in food, and provides a way for exposure estimation and risk assessment.