具有血管紧张素转化酶抑制活性的益生菌筛选鉴定及其发酵特性的研究

Study on separating, identification and fermentation characteristics in probiotics with inhibitory activity on angiotensin converting enzyme

目的筛选具有血管紧张素转化酶(ACE)抑制作用的微生物,提供适用于降高血压发酵产品的菌株。方法从7种发酵食品中分离菌株后,利用体外ACE酶抑制率法筛选ACE抑制率大于80%的菌株,再通过耐盐和耐NaNO2实验,筛选出目的菌,并对其进行生物学特性测定和分子生物学鉴定。结果从发酵食品中分离得到98株菌中,筛选出F2和D2两个目的菌,F2和D2的ACE酶抑制率分别为83.13%和98.00%,且均能耐受6%的NaCl及100mg/kg的NaNO2。结论筛选出的F2菌株为地衣芽孢杆菌,对金黄色葡萄球菌抑菌效果显著;D2为球形芽孢杆菌,具有脂肪酶活性;且F2和D2菌均具有蛋白酶活性。

Objective To screen out microbes which have inhibition effect on angiotensin converting enzyme (ACE) for providing the strains which may decrease the blood pressure. Methods After separated from seven kinds of fermented food, the objective strains whose ACE inhibition rate was more than 80%were screened out by the ex-periments of ACE inhibition in vitro, and the salt tolerance and nitrite tolerance, as well as molecular biology and bi-ological characteristics were determined. Results Ninety-eight kinds of strains from 7 kinds of fermented food were separated and screened. Among them, the ACE inhibition rate of F2 and D2 were 83.13%and 98%, respectively, and they could tolerate 6%NaCl and 100 mg/kg nitrite. Conlusion F2 is Bacillius licheniformis and it has antibacterial effect on Staphylococcus aureus, and D2 is Aneurinibacillus miqulanus and it has lipase activity, and they all have protease activity.