分光光度法测定大豆卵磷脂中磷脂酰胆碱含量

Determination of phosphatidylcholine in soybean lecithin by spectrophotometry

目的建立一种简单、准确的卵磷脂测定方法。方法采用KOH溶液将磷脂酰胆碱中的胆碱游离,加雷氏盐反应后,在波长525nm处,通过分光光度法测定胆碱的量。结果该方法在0.075～0.225mmol范围内线性关系良好,线性方程为Y=3.6457X+0.026,R2=0.9987,方法的回收率在95%～103%之间,相对标准偏差小于1.0%,待测液在2.5h之内稳定,能够满足检测要求。结论本方法具有操作简单、准确性高、稳定性好、抗干扰性强的优点,可以用于卵磷脂产品中磷脂酰胆碱含量的测定。

@@@@Objective To establish a simple and accurate method for determination of phosphatidylcho-line. Methods Free choline was obtained from phosphatidylcholine by alkaline hydrolysis and was deter-mined by spectrohpotometry under the wavelength of 525 nm after reaction with Reinecke’s salt. Results The method showed a good linearity over the range of 0.075m mol^0.225m mol,and the linear equation was Y =3.6457X+0.026 with R2=0.9987. The recoveries was in range of 95%~103%, and the relative standard devia-tions (RSDs) were lower than 1%. The liquid was stable in 2.5h, which can meet the testing requirements. Conclusion This method is simple, accurate, stable, and anti-interference, and it can be used for the determi-nation of phosphatidylcholine from lecithin products.