植物花青苷液质联用方法的分析鉴定

Golden rules of separation and characterization of plant anthocyanins by high pressure liquid chromatography-tandem mass spectrometry

花青苷(anthocyanins)是植物中广泛存在的水溶性色素,在食品加工业中具有重要的地位。花青苷与人体健康密切相关,具有较强的抗氧化、抗炎症、抗微生物、抑制血小板凝聚和抗肿瘤等功效,已作为重要的功能因子而受到广泛的关注。花青苷成分的分析鉴定是花青苷生物活性研究的基础。然而,花青苷种类繁多,结构修饰多样,同时易受环境条件的影响,难以分离鉴定。高压液相色谱-二极管阵列检测-质谱联用技术(HPLC-DAD-MS)是花青苷分析最常规的手段。本文综述了如何利用HPLC-DAD-MS快速有效分离花青苷以及结构解析的一般规律及经验,以期为高附加值资源筛选以及花青苷构效关系的进一步揭示提供重要的化学基础。

Anthocyanins are important water-soluble pigments,which have significant influence in food processing industry.Anthocyanins have anti-oxidant,anti-inflammatory,anti-antimicrobial,inhibition of platelet aggregation and anticancer activity,and have been the focus as important functional ingredients.Separation and identification of anthocyanins are the base for studying their bioactive effects.However,the complexity and variety of anthocyanins make it difficult to characterize the composition of anthocyanins.High pressure liquid chromatography(HPLC) coupled with mass spectrometry has become the standard and most powerful method for routine anthocyanin analysis.This paper reviewed the golden rules for separation and identification of anthocyanins by high pressure liquid chromatography-tandem mass spectrometry(HPLC-DAD-MS),which might provide the chemical evidence for clarification of structure-activity relationship and screening for high value-added resources.