PCR法快速检测三种食源性致病菌的研究

Rapid determination of three food-borne bacterial pathogens using PCR method

目的探讨用PCR方法快速检测食品中沙门氏菌(Salmonella spp.)、金黄色葡萄球菌(Staphylococcus aureus)和志贺氏菌(Shigella spp.)的实用性。方法采用能力验证实验和超市样品检测实验对PCR方法与传统检测方法进行比较。结果与传统检测方法相比,PCR方法检测以上三种食源性致病菌的结果准确率达到99%以上,假阳性率低于1%,假阴性率为0。结论 PCR检测方法具有检测周期短等优点,可以在当前的食品安全检测工作中应用推广。

Objective To rapidly detect Salmonella spp.,Staphylococcus aureus and Shigella spp.in food by PCR.Methods The detection performance of PCR method was compared with that of traditional method by proficiency testing and testing of supermarkets samples.Results The correct rate of PCR method was more than 99%,the false positive rate was less than 1%,and the false negative rate was zero.Conclusion The PCR method is high-effective in testing three food-borne bacterial pathogens,with the advantage of a short detection period,which could be applied in present food safety testing.