4株红曲霉发酵产生次级代谢产物的分析

Analysis of the secondary metabolites produced by 4 strains of Monascus

目的为研究评价红曲霉产生次级代谢产物能力,选育优良菌株。方法通过建立的发酵液中γ-氨基丁酸(GABA)、红曲色素、monacolin K和桔霉素的比色法和高压液相色谱检测方法,比较了紫色红曲霉(Monascus purpureus)、丛毛红曲霉(Monascus pilosus)、红色红曲霉(Monascus ruber)、橙色红曲霉(Monascus aurantiacus)的液体发酵产生上述次级代谢产物能力。结果不同红曲霉菌株产生上述4种次级代谢产物的能力是不同的,4种红曲霉均可产生的红曲色素、monacolin K和桔霉素3种次级代谢产物,其中M.pilosus J2的monacolin K产量最高(8.1 mg/L)、桔霉素生成量最低(0.09 mg/L);M.purpureus Y4的红曲色素色价最高,为380U/mL;仅有紫色红曲霉Y4可代谢产生γ-氨基丁酸,产量约为0.2 g/L。结论不同红曲霉菌株产生γ-氨基丁酸、红曲色素、monacolin K和桔霉素这4种次级代谢产物能力存在较大差异。

Objective To study the ability of secondary metabolites of Monascus, and to breed excellent strains. Methods Detection methods ofγ-aminobutyric acid (GABA), monascus pigments, monacolin K and citrinin by colorimetry and HPLC in fermentation were founded, the abilities of secondary metabolites pro-duced by Monascus purpureus, Monascus pilosus, Monascus ruber and Monascus aurantiacus were compared. Results The producing abilities of secondary metabolites among the 4 strains of Monascus were different. The four strains of Monascus could produce monascus pigments, monacolin K and citrinin. The M. pilosus strain J2 had the highest producing ability of monacolin K (8.1 mg/L) and lowest producing ability of citrinin (0.09 mg/L);M. purpureus Y4 had the highest producing ability of monascus pigments producing (380 U/mL), and only M. purpureus Y4 can produceγ-aminobutyric acid (0.2 g/L). Conclusion There are differences in producingγ-aminobutyric acid, monascus pigments, monacolin K and citrinin among different strains.