AlphaLISA技术在食品安全检测中的研究进展

Research progress of AlphaLISA technology in food safety detection

纳米均相时间分辨荧光免疫法(AlphaLISA)是一种基于纳米微珠的光激化学发光的新型均相检测技术,较传统的酶联免疫吸附测定(ELISA)具有均相、免清洗、敏感度高、特异性强等特点。本文介绍了AlphaLISA的原理和特点,简要总结了近年来国内外AlphaLISA技术的应用研究,重点综述了食品安全领域(包括生物毒素和药物残留)的研究进展,并对此技术的发展前景进行了展望。

Homogeneous time-resolved fluoroimmunoassay (AlphaLISA) is a new homogeneous detection technology based on light stimulating of the chemiluminescence of nano micro-bead. Compared with the tradi-tional ELISA, the AlphaLISA has characteristics of homogeneity, free of cleaning, high sensitivity and speci-ficity. This paper introduced the principles and characteristics of AlphaLISA, briefly summarized the applica-tion research of AlphaLISA technology at home and abroad in recent years, reviewed the research progress of food safety field (including biological toxins and drug residues), and the future development of this technology was also prospected.