中部1/3髌韧带切除后其修复过程中的细胞行为及组织学观察

Cellular Behavior and Histological Observation of Rat Patellar Tendon after Removal of Its Central One-third Part

在大白鼠体内模型中,对实验性切除中部1/3髌韧带后,剩余部分髌韧带早期修复过程中的细胞行为和组织学变化进行了研究。研究表明:髌韧带存在着内在修复能力,在本模型中伤口的修复是从伤口中部开始并向腹侧和背侧延伸。伤口的修复先是伤口周边的细胞移行进伤口,然后到达目的地的细胞开始分泌胶原蛋白,并形成新生的胶原纤维。纤维粘连蛋白在损伤修复的早期存在表达,可能是充当使参与修复的细胞从伤口的边缘移行到伤口中央的生物桥梁。伤口中的细胞和胶原纤维修复到接近组织学正常的时间约需6至9个月。

The use of a central one-third patellar tendon as an autograft for surgical reconstruction of a damaged anterior cruciate ligament is common, yet few complications of its use were reported. In this study the cellular behavior and histology in healing process at the remaining patellar tendon after removal of its one-third part were observed and it was found that the patellar tendon possessed intrinsic healing capabilities. The healing process initiated from the center of the wound side and then progressed toward the dorsal and ventral patellar tendon. At the beginning of healing process, cells migrated from, margin of the wound then to the central area. After they arrived the target place, they would secrete collagen. The new born collagen fiber would be generated by the collagen. It was also found that the fibronectin was expressed in the early stage of healing process, which might function as the bio ?bridge that benefited the cells migrating from the margin of the wound to the central area. It would need about 6 to 9 months before cells and collagen fibers recover in patellar tendon healing.