Nod1受体激活对腹膜间皮细胞自噬相关蛋白表达的影响

Nod1-agonist induced autophagy-related protein expression in rats peritoneal mesothelial cells

目的探讨Nod1受体激活对腹膜间皮细胞自噬的影响。方法 Nod1受体激动剂iE-DAP作用大鼠原代培养腹膜间皮细胞0、1、2h,Western blot检测微管相关蛋白1轻链3(LC3)、Beclin-1的表达。结果随刺激时间延长,LC3-Ⅱ/LC3-Ⅰ蛋白表达比值增加,Beclin-1蛋白表达亦增加。结论 Nod1受体激活可诱导腹膜间皮细胞自噬的发生,这有可能发挥清除细胞内细菌感染的作用。

Objective To investigate the Nod1-agonist-induced autophagy-related protein expression in rats peritoneal mesothelial cells. Methods Rat peritoneal mesothelial cells in the logarithmic growth phase were cultured. Cells were treated with iE-DAP(Nod1-agonist)at 0h,1h and 2h. Western blot analysis were used to detect the expressions of microtubule-associated protein 1 light chain 3(LC3)and Beclin-1 in cell. Results Compared with the control group,the ratio of LC3-Ⅱ/LC3-Ⅰ and Beclin-1 protein expression increased with the prolongation of iE-DAP stimulation. Conclusion Autophagy of rat peritoneal mesothelial cells can be induced by Nod1-agonist, which may enhance the antibacterial capacity.