摘要
采用室内模拟试验,对昆虫病原线虫检测器收集到昆虫病原线虫线虫数量和土壤中昆虫病原线虫密度相关性进行研究,确定昆虫病原线虫检测器收集时间、检测器放置间距进行了试验研究。结果表明,收集时间和检测器放置间距对检测器收集到线虫数量有显著影响,48h为最佳收集时间,检测器之间放置8cm为最佳间距。检测器中收集到昆虫病原线虫的数和昆虫病原土壤中线虫密度显著相关,相关系数R2达到0.9364。该技术为昆虫病原线虫定性及定量检测提供了一种新方法。
laboratory simulation test was used for this research,the relation between population density of EPN in soil and the individual number in detection tube was studied. Collection time and the distance between detection tubes were studied by one way test. The results showed that collection time and the distance between detection tubes affected the individual number in detection tube significantly. 48 hours was the best Collection time and 8cm was the best distance between detection tubes for the EPN collection. The result showed that the individual number in detection tube was related to the population density of EPN in soil ,the highest R2 was 0. 9364. This research provide a new method for qualitative and quantitative of EPN detection.
出处
《土壤与作物》
2013年第2期71-76,共6页
Soils and Crops
基金
宁夏农业综合开发项目(NTKJ2012-05-01)
