棉酚合并卡前列甲酯在小鼠的抗早孕作用及其机理研究

Effect of Gossypol Acetic Acid combined with 15-Methyl PGF_(2α) Methyl Ester on Early Pregnancy in Mice

单用棉酚75mg/kg/d×3天无抗早孕作用，而同样剂量棉酚与PG050．2mg/kg/d×2天合用可显著增强后者的抗旱孕作用，使小鼠活胎数进一步减少；棉酚30μg/ml和（－）棉酚15μg/ml对大鼠黄体细胞3β-HSD活性均有显著抑制作用，而（+）棉酚15μg/ml和PG0510μg/ml对此酶活性均无影响；（－）棉酚15μg/ml还能抑制大鼠颗粒细胞雌二醇的分泌．提示棉酚增强PG05抗旱孕作用可能与它从环节影响胚胎的正常发育有关．

This article observed the pregnancy - interrupting action of Gossypol Acetic Acid(GAA) combined with PG05. the results showed that administration of GAA at 75mg/kg × 3d alonecouldn' t interrupt the pregnancy. however, same dosage of GAA combined with PG05 0.2mg/kg/d ×2d enhanced significantly the interrupting effect of the latter on early pregnancy; GAA 30μg/ml and(-)GAA 15μg/ml inhibited markedly the activity of 3β - HSD of luteal cells in rat, but (+ )GAA15μg/ml and PG05 10μg/ml had no the inhibitory effect on the achtivity of it. (-)GAA 15μg/ml alsoinhbited significantly basal Easal Estradiol production of granulosa in rat. It was concluded that the interrupting effect of PG05 on early pregnancy enhanced by GAA resulted from decrease of prpgesterone production induced by both GAA and PG05.