皱叶酸模总黄酮和多糖的超声提取及含量测定

Title Ultrasouic extraction and determination of chromocor and polysaccharide in Rheum crispus L.

目的:从皱叶酸模中提取总黄酮、多糖,并测定其含量。方法:运用超声技术连续提取皱叶酸模总黄酮、多糖,用比色法测定总黄酮、多糖含量。结果:测得皱叶酸模中总黄酮含量0.32%,平均回收率为98.63%,RSD=1.27%(n=6):多糖含量8.25%,平均回收率为99.78%,RSD=0.93%(n=5)。结论:首次运用超声技术从皱叶酸模中连续提取总黄酮、多糖,速度加快,回收率提高。

Chromocor and polysaccharides were extracted from Rheum crispus L. by ultrasonic method,and the content was determination.Methods:Chromocor and polysaccharides were extracted by ultrasonic mcthod,and the contcnt was determined by spectrophotometer, Results:The chromoeor content of Rheum crispus L. is 0.32%,ricovery rate is 98.63%,The polysaccharide content of Rheum crispus L. is 8.25%,recovery rate is 99.78%。Conclusion:lt is the first time to extract chromocor and polysaccharides from Rheum crispus L. by ultrasonic method,Utrasonic extraction was a better method for chromocor and polysaccharides of Rheum crispus L.。