紫外分光光度法测定毛蕊花糖苷浓度及其油水分配系数

Determination of verbascoside and its oil-water partition coefficient by ultraviolet spectrophotometry

目的：测定毛蕊花糖苷的油水分配系数。方法采用紫外分光光度法测定毛蕊花糖苷的浓度,结合摇瓶法,正辛醇-水系统测定毛蕊花糖苷的油水分配系数。结果在0~30μg/mL范围内,毛蕊花糖苷的浓度与吸光值成线性关系,相关系数为0.999；平均回收率为(102.16±0.703)%, RSD 为0.688%(n=3)；油水分配系数为0.273, logP=-0.566(n=3),说明毛蕊花糖苷易溶于水。结论紫外分光光度法测定毛蕊花糖苷浓度操作简便,准确性好,可作为毛蕊花糖苷油水分配系数测定的方法。

Objective To determine the oil-water partition coefficient of verbascoside. Method Ultra-violet spectrophotometry (UV) was used to determine the content of verbascoside. Shake-flash method, octa-nol-water system was employed to determine the oil-water partition coefficient of verbascoside. Results The content of verbascoside was linearly related to its absorbance values when the content was in the range of 0~30 μg/mL, and the correlation coefficient was 0.999. The average recovery was (102.16±0.703) %, with RSD of 0.688% (n=3). And the oil-water partition coefficient was 0.273, logP=-0.566 (n=3), which indicated that verbascoside could be freely dissolved in water. Conclusion The method for determination of verbascoside is simple and has good correctness in this study. It can be used to determine the oil-water partition coefficient of verbascoside.