高效液相色谱-串联质谱法测定桃仁中10种真菌毒素

Determination of 10 mycotoxins in peach kernel by high performance liquid chromatography-tandem mass spectrometry

目的：建立桃仁中黄曲霉毒素 G2、G1、B2、B1、赭曲霉毒素 A、呕吐毒素、玉米赤霉烯酮、伏马毒素 B1、B2及 T-2毒素10种真菌毒素的高压液相色谱-串联质谱测定法。方法样品经70%甲醇溶液超声提取,用水稀释后经固相萃取柱净化,经 C18色谱柱分离,采用电喷雾离子源在正负离子多反应监测(multi-reaction monitoring, MRM)模式下测定,外标法定量。结果10种真菌毒素的线性关系良好,在低、中、高3个水平的加标回收率在60％~100％之间, RSDs(relative standard deviation)为0.4%~3.7%。结论该方法快速、简便、准确,可用于中药材中真菌毒素的多成分测定。

Objective To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for determination of 10 kinds of mycotoxins (aflatoxinG2, G1, B2 and B1, ochratoxin A, deoxynivalenol, zearalenone, fumonisinsB 1, B2, and T-2 toxin) in peach kernel. Methods After extracted with 70% methonal and purified with solid-phase extraction column, 10 kinds of mycotoxins were analyzed by HPLC-MS/MS with multi-reaction monitoring (MRM) mode. Results These mycotoxins showed a good li-near relationship. The mean recoveries at three spiked concentration levels were in the range of 60%~100%with the relative standard deviation(RSDs) from 0.4%to 3.7%. Conclusion This method is simple and accu-rate, and can satisfy the requirement of multi-mycotoxin analysis in Chinese herbal medicine.