黑龙江省鸭肝炎病毒HD-Ⅰ株的分离与鉴定

Isolation and identification of duck hepatitis virus HD-strain in Heilongjiang Province

从黑龙江省某鸭场疑似鸭病毒性肝炎 (DVH )的发病雏鸭肝等组织中分离到 1株病毒HD Ⅰ株。经病理学检查、电镜观察、鸭胚中和试验、雏鸭保护试验、动物回归试验等鉴定为血清Ⅰ型鸭肝炎病毒 (DHV)。证实引起该鸭场雏鸭发病和死亡的主要原因为DVH所致。用DHV标准R85 95 2毒株多次强化免疫健康鸡 ,获得高效价的鸡抗鸭肝炎超免疫抗体。

A strain of virus, which was suspected to be duck hepatitis virus (DHV), was isolated from the diseased and dead ducks on the duck farm in Raohe County of Heilongjiang Province. It was confirmed by pathological examination, electronic microscopy observation, duck embryo neutralization test and duckling protection test that the virus strain was DHV serotype 1 and had resulted in plenty of ducks to be pathogenetic and dead on the farm. The strain was named DHV HD-strain. After healthy chickens were immunized with the DHV standard strain, the antibody to DHV in chicken serum and yolk was gained. The good control effect was obtained by inoculating the immunized antibody into the ducks infected with DHV.