IMC_(10200)株ALV-J实验诱发禽骨髓性白血病的研究

Pathological observation of myeloid leukosis experimentally induced with IMC_(10200) strain ALV-J

对分离自肉种鸡群亚临床感染的J亚群禽白血病病毒IMC10 2 0 0 株进行了实验感染诱发禽骨髓性白血病的病理学研究。IMC10 2 0 0 株J亚群禽白血病病毒尿囊腔接种 11日龄肉鸡胚和SPF蛋鸡胚 ,孵出后跟踪观察。 9周龄时随机抽检感染鸡 ,感染肉鸡特异引物PCR检测全部为阳性 ;组织病理学观察感染鸡无明显病变。至 2 1周龄时感染肉鸡出现第一例典型骨髓性白血病病例 ;感染SPF蛋鸡未见明显J亚群禽白血病相关病变。

The present study was undertaken to observe experimental myeloid leukosis induced with IMC_(10200) strain ALV-J which was isolated originally from a normal broiler breeder.Specific pathogen free (SPF) leghorn and commercial meat-type chickens were used.11-day-old chick embryos were inoculated with IMC_(10200) strain ALV-J,hatched and reared.When reared to 21 weeks of age, some of experimental birds were selected randomly for PCR diagnosis and pathological observation. All of virus-inoculated meat-type chickens chosen were positive in PCR of H5/H7 but no clear pathologic changes.The first case of ML occurred in meat-type chickens with 42 weeks of age.No tumors were present in SPF leghorns infected.