摘要
为了验证表达猪繁殖与呼吸综合征病毒 (PRRSV)GP5蛋白的重组伪狂犬病病毒 (rPRV_GP5 )的安全性和免疫原性 ,将 10只 5～ 6月龄健康绵羊分成三组 ,安检组 (3只 )肌注 10 8 0 PFU/只、效检组 (4只 )肌注 10 6 0 PFU/只 ,3只作为不接种对照组。接种后 14d ,效检组与对照组每只臀部肌注伪狂犬病病毒“双城系”猪源强毒S株 10 3 LD50 ,结果效检组全部保护 ,对照组全部死亡 ,安检组未见异常。结果表明 。
To test the safety and efficacy of the recombinant pseudorabies virus (PRV) expressing porcine reproductive and respiratory syndrome virus (PRRSV) GP5 (rPRV-GP5) developed earlier against pseudorabies,Ten 5～ 6-month-old sheep were randomly divided into three groups,one group of 4 sheep (efficacy group) were immunized intramuscularly 10^(6.0) PFU,one group of 3 sheep (safety group) were immunized intramuscularly 10^(6.0) PFU,and the third group of 3 sheep were served as control group.Fourteen days post-immunization,animals in efficacy and control groups were challenged by intramuscular inoculation with 10~3 LD50 of the highly virulent PRV S strain of porcine origin.The results showed that vaccinated sheep were protected from lethal challenge of PRV,and rPRV-GP5 represents a candidate vaccine against porcine pseudorabies.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2004年第4期287-289,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
国家 8 6 3计划资助项目 (2 0 0 1AA2 130 5 1)
