甘蔗和斑茅的杂交利用及其杂种后代鉴定系列研究(四)甘蔗与斑茅抗旱生理生化差异性分析

Analysis of the difference of drought tolerance in Saccharum officinarum and Erianthus arundinaceum Retz

进行了水分胁迫及复水过程中斑茅和甘蔗活性氧代谢和渗透调节变化的研究。结果表明,随着水分胁迫程度的提高,斑茅丙二醛(MDA)含量提高幅度低于拔地拉,但超氧化物歧化酶(SOD)、过氧化物酶(POD)比活性较高,同工酶谱带较强(SODb1、SODb2和PODa1)且明显不同于拔地拉,而且其在严重胁迫后复水出现与拔地拉相同的新的诱导谱带PODb2。过氧化氢酶(CAT)比活性分析结果还表明,拔地拉在水分胁迫下,比活性明显下降,而斑茅则里上升趋势。

Changes of osmotic adjustment and active oxygen metabolism of Erithanus arundinaceum and Sacchanun offieinarum (cv. Badila) under water stress were studied. The results indicated that the increment of MDA in Erithanus arundinaceum was less than that in S. offieinarum with the water stress. On the contrary, the specific activities of SOD and POD were both obviously higher than those in Badila. The isozymes (SODb1, SODb2 and PODa1) of Erianthus arundinaceum were stronger and obviously different from those of Badila. In addition, a new isozyme (PODb2), the same as in Badila, was induced in Erianthus arundinaceum when re-watered after severe water stress. The results also showed that CAT specific activity declined rapidly in Badila but increased in Erianthus arundinaceum after water stress, suggesting that Erianthus arundinaceum was more adaptive to water stress and that the drought resistance gene could be cloned from Erianthus arundinaceum.