无菌培养条件下不同碳素和NH_4^+水平对刺线虫生活史的影响

Effect of different organic carbon and NH_4^+ levels on life cycle of Belonolaimus longicaudatus on excised corn root

在24℃无菌、无光照、不同碳素和氮素水平培养条件下,观察了以玉米切根根尖为食的长尾刺线虫 Belono-laimus longicaudatus的发育.结果表明,刺线虫完成生活史所需的最短时间没有明显受到不同碳素水平的影响.碳素缺乏时,刺线虫的存活率和成虫的比率明显下降.在5-10倍于标准B5培养基中NH4+水平的环境下,没有观察到NH4+对刺线虫的发育有不良影响.

The effect of different sucrose and NH4+ levels on development of Belonolaimus longicaudatus was separately investigated on excised corn root in 24℃. The results showed that the maximum development rate of the nematodes was not obviously affected by organic carbon levels tested as nematodes approaching in the stage of adult. Carbohydrate-poor nutritious sediment had an important impact on the survival rate and the percentage of adult of the sting nematode. Whereas, the ammonium-rich amendment didn' t have any negative impact on the maximum rate of development, survival rate, and the percentage of javeniles and adults within the certain range tested.