鸭卵清蛋白5'端调控区的克隆和序列分析

Cloning and sequence analyses of the 5' regulation regions of duck ovalbumine gene

通过PCR技术从产蛋鸭输卵管基因组中扩增出1.2 kb的鸭清蛋白5’端调控区,将其亚克隆入phD18-T载体的多克隆位点(命名为 pOV),经酶切和测序鉴定可作为启动外源基因表达的调控序列.为构建其启动外源基因的质粒表达载体作准备.

5' terminal regulatory region of ovalbumin gene, a 1.2 kb DNA segment, was amplified by PCR from oviduct genome of a laying duck. It was cloned into the multiple cloning site of phD18-T vector (now named pOV), and used as regulatory sequence for promoting exogenous gene expression after enzyme cutting and sequencing identification. It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.