针刺和静力牵张对大负荷运动后骨骼肌收缩结构变化影响的免疫电镜研究

An Immuno-Electron Microscopic Study of the Effect of Acupuncture and Static Stretch on Contractile Structural Alteration of Skeletal Muscle After Strenuous Exercise

用免疫电镜的方法观察大负荷运动后骨骼肌收缩结构变化时M蛋白、α-辅肌动蛋白和肌球蛋白的定位,以探索超过习惯负荷后收缩结构延迟性变化的性质,以及针刺和静力牵张对促进其恢复的机理,结果发现:在多组力竭性工作后,收缩结构发生变化的同时,伴有该结构的免疫标记密度下降以及有关收缩蛋白的免疫标记向其结构以外区域扩散,这一结果提示:超过习惯负荷后收缩结构变化或解体是由于延迟性的收缩蛋白的降解优势所致。由于这种变化是一过性的,经过休息和调整训练负荷即可能恢复,并未形成稳定的改变,因而其性质仍可认为是生理性的或是生理向病理性变化的过渡状态。针刺和静力牵张能通过加强收缩蛋白的合成代谢而有效地促进收缩结构的恢复并出现疼痛的消除,其作用的机理尚待进一步的研究。

Immuno-electron micoscopic observation of the localization of M-protein,α-actinin and myosin was made to study the nature of delayed structural alteration of myofibril and the effective mechanism of oblique acupuncture and static stretch on the structural recovery of skeletal muscle myofibril after strenuous exercise. It was observed that:l. different degree of structural alteration of Z-line and M-line especially the alteration of thick filament after total disruption of M-line were found; 2. delayed structural alteration of myofibril was accompanied by decrease in label density of contractile proteins and diffusion of these proteins to their adjacent area within sarcomere, these results suggested that delayed alteration of contractile structure was the result of the predominant degradation of contractile protein after over exertion, Therefore, the delayed structural alteration of myofibril could be defined as physiological of transitive state from physiological to pathological in nature. The mechanism of acupuncture and static stretch for relieving muscle soreness or pain is not the same as acupuncture anesthesia, However,by enhancing the turnover(anablic process) of contractile protein it promotes the structural recovery of the myofibril and relieves soreness or pain of muscle at the same time.