针刺和静力牵张对大负荷运动后骨骼肌M线变化影响的免疫电镜研究

An Immuno-Electron Microscopic Study of the Effect of Acupuncture and Static Stretch on Ultrastructural Alteration of M-Line of Skeletal Muscle After Strenuous Exercise

用胶体金标记技术定位运动后及运动后经针刺或静力牵张处理的人骨骼肌M蛋白,以探讨超过习惯负荷后骨骼肌收缩结构变化以及针刺和静力牵张对促进其恢复的机制。我们发现:大负荷斜蹲后未经针刺的对照腿股外肌M线的M蛋白标记密度低于针刺腿(P<0.05),而对照腿M线两侧及M线两侧以外的M蛋白标记密度高于针刺腿(P<0.05)。对照实验证明运动腿M线以外增加的标记物为物异性标记。静力牵张组在M线及M线两侧的标记结果与针刺组一致。仅在M线两侧以外区域牵张腿与运动对照腿之间标记密度的差异没有统计学意义。我们还观察到运动后对照腿变化M线体密度达42—48％,而针刺或牵张腿为6—9％。上述结果表明大负荷运动加强了负荷后骨骼肌M蛋白的解聚或降解,从而导致M线结构改变;针刺和静力牵张可显著促进M蛋白的合成或抑制其降解,而使M线结构恢复或稳定。本文还探讨了M线变化与肌粗丝结构变化的关系。

The immuno-electron microscopic method was used to study the nature of ultrastructural alteration of Mline in human skeletal muscle after strenuous exercise and the effect of acupuncture or static strethch on those alterations, It was observed that the label density of M-line of the control thigh was lower than that of the acupunctured, but higher on both sides with equal width of the M-line and areas beyond in the sarcomers. Similar results were observed in the static stretch group,but the difference of label density between the control and the static stretch in the areas beyond M-line was nonsignificant. It was also indicated that acupuncture or static stretch could effectively enhance the synthesis of M-protein and promote the recovery process of the M-line. The volume density of the ultrastructural alteration of-M-line decreasing from 42-48% in the control to 6-9% in the acupunctured or static stretched thigh presented further support to the explanation discribed above.