6个牛品种在FSHR基因位点的遗传关系及其多态对双胎性状的标记

Polymorphism on the 5′ flanking region of FSHR gene in6 bovine breeds and the relationship between itspolymorphisms and the twining traits in 3 bovine breeds

用PCR-RFLP技术,对秦川牛、中国荷斯坦牛、晋南牛、南阳牛、延边牛、中国西门塔尔牛6个牛品种176个个体的促卵泡素受体(FSHR)基因5′端进行多态性分析,4种内切酶对PCR产物的单酶切结果发现,Pst ,Sin 和Hae 3种内切酶的酶切产物均为单态,而Taq 的酶切产物出现多态性,表现为3种基因型(AA,BB,AB),品种间基因频率和基因型频率均有较大差异。对3个有单双胎记录牛的品种分析发现,秦川牛双胎母牛的等位基因A(0.5500)及基因型AA(0.5000)的频率高于单胎母牛(A:0.3500,AA:0.3000);而荷斯坦牛双胎母牛的等位基因B(0.5937)及基因型BB(0.6875)的频率均高于单胎母牛(B:0.5416,BB:0.4167),在后代中有同样的趋势;在中国西门塔尔牛的双胎母牛、随机母牛及种公牛3种类群中,双胎母牛和种公牛的等位基因B(0.5909,0.5417)和杂合子基因型AB(0.8182,0.9167)的频率高于随机母牛(B:0.3182;AB:0.4546)。根据Nei氏标准遗传距离聚类分析,表现为西门塔尔牛和延边牛首先聚在一起,然后晋南牛、秦川牛、荷斯坦牛、南阳牛依次加入。

PCR-RFLPs of 5′-flanking region of the follicle-stimulating hormone receptor (FSHR) gene,which includes exon 1 from 176 cattle of 6 breeds Qinchuan cattle,Chinese Holstein,Jinnan cattle,Nanyang cattle,Yanbian cattle,Chinese simmental,were analyzed with restriction endonucleases PstⅠ,SinⅠ,HaeⅢ and TaqⅠ.The results demonstrated that no polymorphism was found after digestion of PCR products with restriction endonucleases PstⅠ,SinⅠ and HaeⅢ,but 3 genotypes (AA,BB,AB) were found after digestion of PCR products with restriction endonuclease TaqⅠ and there were great differences in frequencies of genes and genotypes among 6 breeds.The relationship between the polymorphism and the twinning traits was further analyzed in 3 bovine breeds.The results indicated that the frequencies of allele A (0.550 0) and genotype AA (0.500 0) in twinned cows were higher than those of allele A (0.350 0) and genotype AA (0.300 0) in monovular cows for Qinchuan cattle,while the frequencies of allele B (0.593 7) and genotype BB(0.687 5) in twinned cows were higher than those of allele B (0.541 6) and genotype BB (0.416 7) in monovular cows for Holstein cattle.In 3 populations of Chinese Simmental,the frequencies of allele B and genotype AB were higher in twinned cow and seed bull than in the control cattle.Based on frequencies of the alleles of 6 cattle breeds to estimate the Nei-standard genetic distances among 6 breeds,the phylogenetic tree showed that Chinese Simmental and Yanbian cattle would cluster together first,than Jinnan cattle,Qinchuan cattle,Holstein and Nanyang cattle would be joined in turn.