丛枝菌根真菌的增殖技术

Propagation Technology of AMF

对目前国内丛枝菌根真菌的主要增殖方法进行比较研究 .以三叶草作宿主植物 ,采用单孢和多孢两种接种方法 ,分别将摩西球囊霉、地表球囊霉、地球囊霉、缩球囊霉和网状球囊霉 ,在土培、沙培、水培的条件下进行增殖 .收获后分别测定它们的侵染株率、侵入点的密度和侵染率 .研究结果表明 ,三叶草是理想的宿主植物 ,其在液培条件下丛枝菌根真菌侵染能力最高 ,沙培法法次之 ,土培法最低 。

A comparative study is made on the main propagation technology of Arbuscular mycorrhizal Fungus (AMF) in domestic labs at present. Take clove ( Trifolium pratense ) as host plant and adopt single spore and multi spore methods of inoculation. Let G. mosseae, G. versiforme, G. geosporum, G. constrictum and G. reticulatum be multiplied under the conditions of soil, sand and nutrition liquid cultivation respectively. After harvest, determine respectively the rate of plants infected, the density of infection points and the infection rate. As shown by results of research, clove is an ideal host plant; the infection ability of AMF is the highest under sand caltivation liquid cultivation, the second under sang cultivation, and the last under soil cultivation; G. mosseae and G. versiforme have higher infection ability.