口蹄疫OX株全基因组cDNA的分子克隆、序列测定及分析

Molecular Cloning and Sequence Analyzing for the Full-length Genome of Foot-and-Mouth Disease Virus OX strain

采用RT-PCR法对FMDVOX株基因组全序列进行了分子克隆与测序。结果表明不含poly(C)序列和poly(A)尾巴的OX株基因组全序列长8104nt,开放读码框(ORF)长6969nt、编码2322aa,5′UTR长1040nt,3′UTR长95nt。应用分子生物学软件将OX株与FMDV其它参考毒株进行序列比较,并对其基因特征进行分析。结果显示,OX株与OTwyl/97的核苷酸同源性最高,在基因组功能未知区和3A编码区具有两处明显的缺失现象,其一是在3A编码区有30nt的连续缺失,这与OTwyl/97株相同,其二是在功能未知区域有44nt的缺失,这与OTwyl/97株相同,与OAkesu58相似(43nt缺失)。

The complete sequence of foot-and-mouth disease virus (FMDV) OX strain was acquired by RT-PCR. Then all regions of OX strain genome were compared with those of the reference strains which sequences were obtained from GenBank, and their genetic characteristic were analyzed by molecular biological software. As a result, the genome of FMDV OX strain is 8 104nt without poly(C) and poly (A), and is composed of 5′untranslated region (UTR), open-reading frame (ORF) and 3′untranslated region (UTR), their nucleotide is 1 040nt, 6 969nt and 95nt respectively. The OX strain has the most homologous with OTWyl/97 and there are two distinct genetic deletions in function-unknown region and 3A encoding region, one is a 30nt sequential deletion in 3A encoding region which is same as OTWyl/97 strain, the other is a 44nt sequential deletion in unknown-functional region which is same as OTWyl/97 strain and similar to OAkesu58 (43nt deletion) strain.