脂质体为模板仿生硅化固定葡萄糖氧化酶

Immobilization of glucose oxidase in liposome-templated biomimetic silica particles

将脂质体囊泡与仿生硅化技术相结合,模拟细胞纳微环境,实现以脂质体为模板仿生制备氧化硅固定葡萄糖氧化酶(GOx),建立性能稳定的固定化酶.扫描电镜分析显示,固定化GOx为球形纳米粒子,粒径分布在200nm左右,在优化反应条件下GOx回收率达到71.8%.由于载体的空间限制作用及其提供的较稳定微环境,固定化GOx表现出良好的热稳定性和pH稳定性,其对变性剂耐受性和操作稳定性等也得到明显提高.

Glucose oxidase (GOx) was immobilized in biomimetic silica particles by coupling liposome vesicles with a biomimetic silicification process simulating a cell microenvironment. SEM images showed that the immobilized GOx particles were spherical, and their diameter was approximately 200 nm. The recovery of GOx reached 71.8% under the optimized reaction conditions. The immobilized GOx showed improved thermal and pH stability, and its tolerance to denaturants and reusability were also greatly improved. This was attributed to the space limitations and stable environment in the support.