超高效液相色谱法测定乌药中去甲异波尔定的含量

Determination of Norisoboldine in Radix Linderae by UPLC

目的建立乌药中去甲异波尔定含量测定的超高效液相色谱分析方法,为改进现行《中国药典》标准中乌药质控方法奠定基础。方法采用ACQUITY UPLC BEH C18 column(50 mm×2.1 mm,1.7μm)色谱柱,以乙腈-甲酸溶液(0.5%,v/v,加三乙胺调节pH至2.25)为流动相,梯度洗脱,检测波长为280 nm,柱温30℃。结果去甲异波尔定在0.3130～313.0 ng峰面积与进样量呈良好的线性关系,r为0.999 8;平均回收率为97.68%,相对标准偏差为2.13%。结论本方法简便、快捷、准确,可作为一种替代方法用于日常样品的高通量检测。

Objective To establish a UPLC method for the determination of norisoboldine in Radix Linderae and lay a foundation for the improvement of quality control method of Radix Linderae in Chinese Pharmacopoeia.Methods A UPLC was performed on ACQUITY UPLC BEH C18 column(50 mm×2.1 mm,1.7 μm) with gradient elution of acetonitrile-formic acid solution as mobile phase(adjusted pH to 2.25 with triethylamine),the detective wavelength was 280 nm,and the column temperature was 30℃.Results The linear range of norisoboldine was 0.3130～313.0 ng(r=0.9998),the average recovery was 97.68%,and the relative standard deviation was 2.13%.Conclusion The method is simple,rapid and accurate,and can be used as an alternative method for high throughput detection of daily samples.