HPLC法测定双藤痹痛凝胶膏剂中雷公藤甲素和青藤碱的含量

Determination of Triptolide and Sinomenine in Shuangteng Bitong Cataplasm by HPLC

目的控制双藤痹痛凝胶膏剂的质量。方法用HPLC法测定双藤痹痛凝胶膏剂中雷公藤甲素和青藤碱的含量。结果雷公藤甲素的线性范围为1.5216~15.2160μg/mL,平均回收率为98.07%;青藤碱的线性范围为8.5524~85.5240μg/mL,平均回收率为98.32%。结论 HPLC法操作简便快速,结果准确,重现性好,可用于双藤痹痛凝胶膏剂的质量控制。

Objective To control the quality of Shuangteng Bitong cataplasm.Methods The contents of Triptolide and Sinomenine in Shuangteng Bitong cataplasm were determined by HPLC.Results The linear range of Triptolide was 1.5216-15.2160 μg·mL-1,and the average recovery was 98.07%.The linear range of Sinomenine was 8.5524-85.5240 μg·mL-1,and the average recovery was 98.32%.Conclusion This method is simple,rapid,accurate,reproducible,and could be used for the quality control of Shuangteng Bitong cataplasm.