阿氏节杆菌菌株DA-1的厌氧反硝化研究

Anaerobic Denitrification Characteristics of Arthrobacter arilaitensis DA-1

阿氏节杆菌(Arthrobacter arilaitensis)菌株DA-1是一株氨氮降解细菌,且在有氧及厌氧条件下均具有反硝化作用。通过对该菌的厌氧反硝化条件进行优化,考察了碳源、C/N、温度和初始pH对其反硝化作用的影响。结果表明,乙酸钠为最佳的反硝化碳源,当C/N为10∶1、温度为30℃、初始pH为7.0时,A.arilaitensis DA-1能获得最高的反硝化效率,在该条件下,向硝酸盐模拟污水(NO3--N浓度为25mg/L)中接入2%(V/V)的A.arilaitensis DA-1菌剂,经过48 h的静置培养,NO3--N的去除量达到了20.13 mg/L,而此时NO2--N积累浓度仅为0.56 mg/L。

Arthrobacter arilaitensis DA-1 was an ammonia degradation strain,and showed denitrification ability under both aerobic and anaerobic conditions.The effects of carbon source,C/N,temperature and initial pH on anaerobic denitrification of A.arilaitensis DA-1 were investigated.The results showed that acetate was the best carbon source.When C/N,temperature and initial pH was 10∶1,30 ℃,and 7.0,respectively,the highest denitrification efficiency of A.arilaitensis DA-1 was obtained.Under the optimal conditions,inoculating A.arilaitensis DA-1 to simulated nitrate sewage containing 25 mg/L NO3-N with dose of 2%(V/V),after static culture of 48 h,the removal dose of NO3--N reached to 20.13 mg/L,while the accumulation of NO2--N was only 0.56 mg/L.