摘要
探究NaCl胁迫下小桐子(Jatropha curcas)幼苗叶片蛋白质的变化。以小桐子幼苗叶片为材料,用去离子水、10%的NaCl、0.2%的NaOH、70%的乙醇提取蛋白质,用SDS-聚丙烯酰胺凝胶电泳检测活性蛋白质的分布及差异。结果表明,胁迫时间12 h,40、60 mmol/L NaCl胁迫小桐子,幼苗叶片的蛋白质变化最明显。
To study the change of protein in the leaf of Jatropha curcas seedlings under salt stress,protein was extracted separately from leaves of J.curcas seedlings by distilled water,10% NaCl solution,0.2% NaOH solution and 70% C2H5OH solution.The distribution of the protein was detected by SDS-polyacrylamide gel electrophoresis.The result showed that the change of protein in seedling leaves was the most obvious if treated with 40 mmol/L and 60 mmol/L NaCl for 12 h.
出处
《湖北农业科学》
北大核心
2013年第1期111-114,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(31060095)
海南师范大学植物学学科建设基金项目(HS-1-2011-071001)
2010年度国家大学生创新性实验计划资助项目(101165831)