天门冬组培苗生根的影响因素研究

Influencing Factors on Rooting of Tissue Culture Seedling of Asparagus cochinchinensis

为了建立有效的天门冬(Asparagus cochinchinensis)组培苗生根系统,分别考察了生长素浓度配比、基本培养基和蔗糖浓度对天门冬组培苗不定根形成的影响。结果表明,培养基中营养元素、激素及蔗糖浓度过高或过低均不利于天门冬组培苗生根。以FeSO4.7H2O浓度降低到6.95 mg/L、含Na2.EDTA 9.33mg/L的1/2MS为基本培养基,添加0.5 mg/L IBA、0.5 mg/L IAA以及20 g/L蔗糖时生根效果最好,生根率为96%。

In order to establish an effective rooting system of tissue culture seedling of Asparagus cochinchinensis,the effects of auxin concentration,sucrose and different basic medium on rooting of tissue culture seedling were studied.The results showed that too high or too low content of nutrition element,auxin and sucrose was not benefit for the rooting of tissue culture seedling.When using 1/2MS with FeSO4·7H2O 6.95 mg/L and Na2·EDTA 9.33 mg/L as the basic medium,supplementing with 0.5 mg/L IBA,0.5 mg/L IAA and 20 g/L sucrose,the rooting effect was the best,with rooting rate as high as 96%.