紫红肉火龙果果皮色素提取工艺的响应面法优化

Optimization of Extraction Conditions for Pigments in the Pulp of Red Pitaya (Hylocereus polyrhizus) Peel by Response Surface Methodology

在单因素试验基础上,选择提取时间、乙醇体积分数、料液比、pH为影响因素,以紫红肉火龙果[Hylocereus polyrhizus(Weber)Britton&Rose]果皮色素提取液吸光度为响应值,应用中心组合(Box-Behnken)试验设计建立数学模型,进行响应面分析,以优化提取工艺。结果表明,紫红肉火龙果果皮色素提取的最佳工艺条件为提取时间51 min、乙醇体积分数32%、料液比1∶45(m/V,g∶mL)、pH 5.3。

Response surface methodology was applied to optimize the conditions for extracting pigment from red pitaya(Hylocereus polyrhizus) peel.Based on single factor experiments,a mathematical model describing the effects of extraction time,ethanol concentration,material to liquid ratio and pH on pigment extraction yield was created using Box-Behnken experimental design and analyzed by response surface methodology.The optimal extraction conditions were found to be extraction time of 51 min,ethanol concentration of 32%,the ratio of material to solvent of 1∶45(m/V,g∶mL) and pH 5.3.