高黎贡山土壤低温淀粉酶产生菌的筛选

Screening of Cold-Active Amylase-Producing Strain from Soil in Gongshan of Gaoli

为获取工业生产上有潜在应用价值的低温淀粉酶产生菌,从高黎贡山百花岭南斋公房采集的土样中定向筛选得到10株产淀粉酶菌株,选择其中透明圈有效值最大的GD-6菌株进行摇瓶发酵。结果表明,该菌株最适产酶温度为20℃,最佳产酶pH为7.0,160 r/min摇瓶培养24 h后,产酶活性为420.9IU/(mL·min)。

In order to obtain cold-active amylase-producing strain with potential value for industrial production,10 amylase-producing strains were isolated from Southern Zhaigongfang of Gongshan in GaoLi.The strain,GD-6,which had effective value for maximum of transparent circle,was chose to ferment.The results showed that the optimal enzyme-producing condition of this strain was as follows: 20 ℃,pH 7.0,160 r/min shake flask for 24 h.Under this optimal condition,the amylase activity was 420.9 IU/(mL·min).