混菌固态发酵产纤维素滤纸酶条件的研究

Cellulase-Producing Conditions by Solid-State Fermentation of Mixed Strains

以稻草粉和麸皮为主要原料,利用拟康氏木霉(Trichoderma pseudokoningii)和黑曲霉(Aspergillusniger)(1∶1)混菌固态发酵产纤维素滤纸酶。以初始pH、发酵时间、发酵温度、接种量、装样量、含水量为研究因子,通过对拟康氏木霉和黑曲霉(1∶1)混菌固态发酵产纤维素滤纸酶的最佳发酵条件进行优化,优化后的最佳产酶条件为:固体培养基含水量为50%,初始pH 5.0,装样量15 g/500 mL,接种量为8%,30℃恒温培养5 d。

Cellulase were produced by solid-state fermentation taking straw and wheat bran as the main raw materials by mixed strains Trichoderma pseudokoningii and Aspergillus niger with the ratio of 1∶1.The fermentation conditions such as the initial pH,fermentation time,cultivation temperature,inoculation dose,medium dose and water content were aptimized by orthogonal experiment.The results showed that the optimized producing conditions of cellulose were,water content in medium,50%;initial pH 5.0;medium dose,15 g/500 mL;inoculation dose 8%;culture temperature,30 ℃;fermenting for 5 d.