摘要
采用正交试验筛选山豆根多糖的最佳提取工艺。结果表明,山豆根多糖的最佳提取工艺为料液比1∶10(m/V,g∶mL),提取2次,提取时间90 min,此条件下山豆根多糖提取率为3.62%。该方法简便、准确、灵敏度高,适用于山豆根多糖的提取。
Orthogonal experimental design was used to optimize the extraction technology of polysaccharides from Sophora tonkinensis Gapnep..The content of total polysaccharides was determined by phenol-vitriolic colorimetry.The optimum extraction technology of polysaccharide was as followed,solid to liquid ratio,1∶10(m/V,g∶mL);extraction time,90 min;extracting twice.The content of polysaccharide was 3.62%.The method was simple,accurate,sensitive and suitable for extraction polysaccharide from S.tonkinensis.
出处
《湖北农业科学》
北大核心
2013年第4期898-899,902,共3页
Hubei Agricultural Sciences
基金
江西省自然科学基金项目(2008GZY0074)
关键词
山豆根
多糖提取
正交试验
Sophora tonkinensis Gapnep.
polysaccharides extraction
orthogonal experiment
