摘要
介绍了昆虫材料扫描电子显微镜观察的主要技术环节及注意事项。扫描电镜观察主要包括材料的预处理、清洗、二重固定、脱水、置换、干燥、粘台、喷金镀膜和观察等步骤。并通过与实例图片的对比,总结和讨论了获得较好观察效果的关键步骤。该技术具有推广价值和借鉴意义。
The main technical aspects and notes of the observation on insect material with scanning electron microscope(SEM) were introduced.The main process of observation by SEM includes pretreatment of the material,double fixation,clean,dehydration,replacement,drying,glue station,spraying metal coating and observation.Through the contrast of experimental pictures,the key technology for obtaining good observation effect was summarized and discussed.The observation technology has wide popularization value and reference significance.
出处
《湖北农业科学》
北大核心
2013年第5期1064-1065,1070,共3页
Hubei Agricultural Sciences
基金
河南科技大学实验室开放基金专项(SY0910059)
国家公益性行业(农业)科研专项(201003025)
关键词
昆虫材料
扫描电子显微镜
显微观察
insect material
scanning electron microscope(SEM)
microscopic observation
