红富士苹果芽变优系的SSAP分析

SSAP Analysis of Red Fuji Apple Bud Mutation

采用SSAP分子标记技术对田间自然产生的红富士苹果(Malus domestica)新芽变材料及其对照进行了遗传鉴定。结果表明,一个376 bp DNA片段为芽变材料特异性片段,其序列与苹果查尔酮合成酶(CHS)基因启动子同源性达90%,表明芽变材料是遗传物质发生改变的稳定变异,为进一步研究芽变材料发生性状变异的分子机理奠定基础。

Fuji apple bud mutation and the wild type were analyzed by SSAP.The results showed that a DNA fragment of 376 bp in length was specific fragment of Fuji apple bud mutation,and it was up to 90% homology with the promoter of chalcone synthase(CHS) gene.It indicated that Fuji bud mutation was stable mutation of the genetic material changes,and laid a basis for further research on the molecular mechanism of bud mutation.