摘要
以小麦(Triticum aestivum Linn.)为材料,以不同浓度梯度的叠氮钠溶液处理小麦叶片后,采用彗星实验研究叠氮钠对小麦的遗传损伤。结果表明,叠氮钠能引起小麦基因的损伤,并随浓度的增加伤害程度加大,表明叠氮钠有明显的遗传损伤毒性,彗星实验可以利用植物细胞快速检测环境中对遗传物质有毒性的物质,是一种快速、简单、直接检测DNA损伤的手段。
Comet assay was conducted to study the genetic damage of NaN3 on wheat(Triticum aestivum Linn.) using wheat leaves treated with different concentrations of NaN3 as material.Experimental results showed that total nitrogen sodium could cause damage on wheat gene;and the damage degree increased with the increase of NaN3 concentration,indicating that NaN3 had obvious genetic damage toxic.Comet assay could detect toxic matters on genetic materials in environment by plant cells rapidly.It was an effective,simple and direct detection measure for DNA damage.
出处
《湖北农业科学》
北大核心
2013年第7期1502-1504,共3页
Hubei Agricultural Sciences
基金
河南省南阳市科技攻关计划项目(2007S1419)
