Fe^+注入诱变莲花突变体的POD和SOD同工酶分析

POD and SOD Isozymes Analysis of Lotus Mutants Induced by Fe^+ Ion Implantation

以经Fe+注入诱变的白洋淀红莲(Nelumbo nucifera var.Baiyangdian)突变体幼嫩叶片为材料,提取过氧化物酶(POD)同工酶和超氧化物歧化酶(SOD)同工酶,对莲花POD和SOD同工酶进行聚丙烯酰胺凝胶电泳,检测不同莲花突变体POD和SOD同工酶酶谱的差异。结果表明,与对照相比,离子注入诱变后的白洋淀红莲不同突变体间POD和SOD同工酶酶谱发生了改变,在Rf为0.09处,M4、M6突变体同时出现了特异性POD同工酶酶带;在Rf为0.19处,M3、M5突变体同时出现了特异性POD同工酶酶带;在Rf为0.33处,M2、M3、M4、M5突变体同时出现了特异性的POD酶带;在Rf 0.55和0.63处,M1、M2突变体同时出现了POD酶带;在Rf为0.41处,M1、M2、M4、M5、M6突变体同时出现了特异性SOD同工酶酶带。离子注入诱变获得的不同莲花突变体可以用POD和SOD同工酶进行鉴定。

The soluble POD and SOD isozyme solutions were extracted from young tender leaves of the Baiyangdian red lotus mutants induced by Fe+ ion implantation in this experiment.To detect POD and SOD isozymes’ difference among the mutants,the mutants were analyzed by polyacrylamide gel electrophoresis.The results indicated that,compared with the control,the zymography of POD and SOD isozymes had been changed among the Baiyangdian red lotus mutants;with Rf value of 0.19,M4 and M6 mutants appeared specific POD enzyme band;with Rf value of 0.33,M2,M3,M4 and M5 mutants appeared specific POD enzyme band;with Rf value of 0.55 and 0.63,M1 and M2 appeared specific POD enzyme band;With Rf value of 0.41,M1,M2,M4,M5 and M6 mutants simultaneously appeared specific SOD enzyme band.Therefore,the POD and SOD isozymes could be used to identify the different lotus mutants.