摘要
采用水提-醇沉法提取野生仙人掌中的多糖,用邻二氮菲-金属铁离子-H2O2体系法、DPPH法、邻苯三酚自氧化法分别研究了仙人掌多糖对羟自由基、DPPH自由基、超氧阴离子的清除作用。结果表明:仙人掌多糖能够有效地清除羟基自由基、DPPH自由基、超氧阴离子,最佳清除率分别为66.91%、67.17%、68.59%,并且其浓度与抗氧化活性呈现正比量效关系。试验证明了野生仙人掌中的多糖具有较好的抗氧化活性。
The polysaccharides in Opuntia dillenii Haw.were extracted by hot water and were precipitated with ethanol.The eliminating effects of Opuntia dillenii Haw.polysaccharides(ODPs) on hydroxy radical,DPPH and superoxide anion were researched by the system of orthophenanthroline-Fe2 +-H2O2,the DPPH method and the Pyrogallol autoxidation method,respectively.The results showed that ODPs can eliminate hydroxy radical,DPPH and superoxide anion effectively with the best eliminating rate of 66.91%,67.17% and 68.59%,respectively,and the concentration and the antioxidant activity of ODPs showed a positive dose-effect relationship.Therefore,the polysaccharides from wild Opuntia dillenii Haw.have great antioxidant activity.
出处
《湖南农业科学》
2013年第2期88-90,共3页
Hunan Agricultural Sciences
基金
湛江市科技计划项目(湛科〔2011〕79号)
关键词
仙人掌多糖
邻苯三酚自氧化
自由基
抗氧化活性
Opuntia dillenii Haw.polysaccharides
Pyrogallol autoxidation
free radicals
antioxidant activity
