2011年湖南省水稻矮缩病病原的RT-PCR检测和病害分级

RT-PCR Detection of the Pathogens for Rice Dwarf Disease in Hunan Province in 2011 and Its Disease Grade

为明确湖南省水稻矮缩病的毒源种类,从湘北常德、湘中长沙和湘南永州三地采集中晚稻水稻秧苗、植株、稻谷等样品,并对其进行了毒源的RT-PCR检测。结果表明:湖南省水稻矮缩病毒源为南方水稻黑条矮缩病毒,所检测样品中没有齿叶矮缩病毒和水稻黑条矮缩病毒。2011年采集的所有水稻秧苗、植株和稻谷样品中南方水稻黑条矮缩病毒检出样品数占有病害症状样品数的23.73%。可以把样品是否具有蜡泪,作为判断南方水稻黑条矮缩病的1个典型症状。其病害危害程度可分为0～4级。

In order to confirm the pathogen type of the rice dwarf disease in Hunan province,the samples of seedling,plant and paddy of middle-late rice were collected from north Hunan(Changde),middle Hunan(Changsha) and south Hunan(Yongzhou) and were detected by RT-PCR.The results showed that the pathogen of the rice dwarf disease in Hunan province was confirmed as south rice black-streaked dwarf virus(SRBSDV),and there was no rice ragged stunt virus(RRSV) or rice black streaked dwarf virus(RBSDV) in the samples.The proportion of the samples with SRBSDV accounted for 23.73% of all the samples with disease symptoms in 2011.The wax tears can be taken as a typical symptom to judge the south rice black-streaked dwarf disease.The south rice black-streaked dwarf disease can be divided into five grades(0-4).