菜薹小孢子培养诱导胚状体的影响因素研究

Influencing Factors for Embryoid Induction of Microspore Culture of Brassica campestris

以8个不同基因型菜薹品种为试材,对影响小孢子培养出胚率的主要因素及胚状体植株再生的条件进行了研究。结果表明:材料的基因型对出胚的影响很大,红菜薹以H09-1的出胚率最高,为1.11个胚/蕾,白菜薹以B09-1最高,为0.514胚/蕾;最适宜的小孢子培养浓度为2×105个/mL;菜薹小孢子培养最佳的胚状体诱导培养基激素组合为1.0 mg/L 6-BA+0.4 mg/L NAA;胚状体植株成苗率最高的培养基琼脂含量以8~10 g/L为宜。

Taking eight different genotypes of Brassica campestris cultivars as materials, the main influencing factors for embryoid induction rate of microspore culture and regeneration of embryoid plant were studied. The results showed that the genotype influenced the embryoid induction rate greatly, the highest embryoid induction rate was H09-1 for Brassica campestris L. ssp. Chinensis var. utilis Tsen et Lee. by 1.11 embryoid/bud, and was B09-1 for flowering chinese cabbage by 0.514 embryoid/bud; the optimum concentration for microspore culture was 2 ×105 spores/mL; the optimal hormone combination for embryoid induction medium for flowering chinese cabbage spores was 1.0 mg/L 6-BA+0.4 mg/L NAA; the optimum agar content in culture medium with the highest embryoid plant seedling rate was 8~10 g/L.