高效液相色谱—紫外法测定水产品中四环素类、喹诺酮类抗生素残留

Determination of Tetracycline and Quinolone Antibiotics Residues in Aquatic Products by HPLC-UV

为了建立高效液相色谱测定水产品中四环素类、喹诺酮类抗生素多组分残留的方法,样品经酸化乙腈提取,正己烷脱脂净化,高效液相色谱分离,紫外检测器检测,外标法定量。结果表明:6种抗生素在0.01~2.00μg/mL范围内线性关系良好,最低检出限为2.00~8.00μg/kg。添加水平为10、20、50μg/kg时,回收率达60.4%~87.7%,RSD≤8.2%。该方法简便、准确,可用于水产品中四环素类、喹诺酮类6种抗生素的同时测定。

In order to establish a HPLC method to determine tetracycline and quinolone antibiotics residues in aquatic products, the samples were extracted by acidified acetonitrile, degreased and purified by n-hexane, and separated by HPLC, detected by UV detector, and then be conducted quantity by external reference method. The results showed that six antibiotics showed a good linear correlation within 0.01~2.00 μg/mL, and the minimum detection limit was 2.00~8.00 μg/kg. When adding level was at 10, 20 and 50 μg/kg, the recovery reached 60.4%~87.7% with RSD≤8.2%. The method is simple and accurate, therefore can be used for simultaneous determining six tetracycline and quinolone antibiotics in aquatic products.