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热休克蛋白60在激活的少突胶质前体细胞的表达

Expression of HSP60 in Activated OPC
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摘要 目的研究热休克蛋白60(HSP60)在重组人干扰素-γ(IFN-γ)和重组人热休克蛋白60(rhHSP60)激活的少突胶质前体细胞中的表达情况。方法分离SD大鼠的少突胶质细胞前体细胞(OPC),经IFN-γ和rhHSP60激活后,应用western blot方法检测细胞中的HSP60蛋白的表达;采用ELISA方法检测细胞培养液中炎症因子TNF-α及IL-6的释放量。结果经IFN-γ和rhHSP60分别激活后,OPC胞体内的HSP60蛋白表达均显著升高,胞外TNF-α及IL-6的释放量也明显增加。结论在激活状态下,OPC中的HSP60蛋白表达显著增高,刺激细胞产生大量炎症因子,从而参与细胞的应激反应。 Objective To explore the expression of HSP60 in IFN- γ and rhHSP60 activated OPC. Methods After OPC were treated with IFN- γ and rhHSP60,cells were collected for western- blot. The release of TNF- α and IL- 6 were detected by ELISA in the supernatant. Results In IFN- γ and rhHSP60 activated OPC,HSP60 protein significantly increased and stimulated the production of amount of cytokines such as TNF- α and IL- 6 compared to control. Conclusion In activated OPC,HSP60 highly expressed to participate the stress response.
作者 李云鸿 齐奇 滕鹏 成文静 马骄 张楠 丁斐嘉 李凡 苗珍花 黄卫东 沈颖 王银
机构地区 宁夏医科大学 浙江大学医学部神经生物学系
出处 《宁夏医科大学学报》 2013年第9期960-963,948,共5页 Journal of Ningxia Medical University
基金 国家自然科学基金项目(31260243 31060140) 教育部新世纪优秀人才支持计划和留学回国人员基金
关键词 热休克蛋白60 少突胶质细胞前体细胞 重组人干扰素-γ 重组人热休克蛋白60 HSP60 OPC IFN-γ rhHSP60
分类号 R363 [医药卫生—病理学]
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参考文献10

  • 1曹智,马骏,袁文俊,林丽.热休克蛋白60与细胞凋亡[J].生理科学进展,2008,39(3):267-270. 被引量：24
  • 2S. R. Kirchhoff,S. Gupta,A. A. Knowlton.Cytosolic Heat Shock Protein 60, Apoptosis, and Myocardial Injury[J]Circulation: Journal of the American Heart Association,2002(24).
  • 3Veereshwarayya, Vimal,Kumar, Pravir,Rosen, Kenneth M.,Mestril, Ruben,Querfurth, Henry W.Differential effects of mitochondrial heat shock protein 60 and related molecular chaperones to prevent intracellular β-amyloid-induced inhibition of complex IV and limit apoptosisJournal of Biological Chemistry,2006.
  • 4Deng Wenbin,Pleasure Jeanette,Pleasure David.Progress in periventricular leukomalaciaArchives of Neurology,2008.
  • 5Gupta Sanjiv,Knowlton A A.HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathwayAmerican journal of physiology Heart and circulatory physiology,2007.
  • 6Gupta S,Knowlton A A.HSP60, Bax, apoptosis and the heartJournal of cellular and molecular medicine,2005.
  • 7Lin Li,Kim S C,Wang Yin,Gupta S,Davis B,Simon S I,Torre-Amione G,Knowlton A A.HSP60 in heart failure: abnormal distribution and role in cardiac myocyte apoptosisAmerican journal of physiology Heart and circulatory physiology,2007.
  • 8Deocaris Custer C,Kaul Sunil C,Wadhwa Renu.On the brotherhood of the mitochondrial chaperones mortalin and heat shock protein 60Cell Stress and Chaperones,2006.
  • 9Stephen A. Back,Byung Hee Han,Ning Ling Luo,Charlene A. Chricton,Steve Xanthoudakis,John Tam,Kara L. Arvin,David M. Holtzm.Selective Vulnerability of Late Oligodendrocyte Progenitors to Hypoxia-IschemiaThe Journal of Neuroscience,2002.
  • 10Vartanian T,Li Y,Zhao M,et al.Interferon-yInduced Oligodendrocyte Cell Death:Implications for the Pathogenesis of Multiple SclerosisMolecular Medicine,1995.

二级参考文献10

  • 1Chang AY, Chan JY, Chou JL, et al. Heat shock protein 60 in rostral ventrolateral medulla reduces cardiovascular fatality during endotoxaemia in the rat. J Physiol, 2006, 574 (Pt 2) : 547 -564.
  • 2Veereshwarayya V, Kumar P, Rosen KM, et al. Differential effects of mitochondrial heat shock protein 60 and related molecular chaperones to prevent intracellular β-amyloid -induced inhibition of complex IV and limit apoptosis. J Biol Chem, 2006, 281:29468 -29478.
  • 3Lin L, Kim SC, Wang Y, et al. HSP60 in heart failure: abnormal distribution and role in cardiac myocyte apoptosis. Am J Physiol Heart Circ Physiol, 2007, 293 : H2238 - H2247.
  • 4Alard JE, Dueymes M, Youinou P, et al. Modulation of en- dothelial cell damages by anti-Hsp60 autoantibodies in systemic autoimmune diseases. Autoimmun Rev, 2007, 6 : 438 -443.
  • 5Carmody RJ, Chen YH. Nuclear factor-kappaB: activation and regulation during toll-like receptor signaling. Cell Mol Immunol, 2007, 4 : 31 -41.
  • 6Gupta S, Knowlton AA. HSP60, Bax, apoptosis and the heart. J Cell Mol Med, 2005, 9 : 51 -58.
  • 7Gupta S, Knowlton AA. HSP60 trafficking in adult cardiac myocytes : role of the exosomal pathway. Am J Physiol Heart Circ Physiol, 2007, 292 : H3052 - H3056.
  • 8Deocaris CC, Kaul SC, Wadhwa R. On the brotherhood of the mitoehondrial chaperones mortalin and heat shock protein 60. Cell Stress Chaperones, 2006, 11 : 116 - 128.
  • 9Cappello F, Czarnecka AM, Rocca GL, et al. Hsp60 and Hsp10 as antitumor molecular agents. Cancer Biol Ther, 2007, 6 : 487 - 489.
  • 10Chan JY, Cheng HL, Chou JL, et al. Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling, and depresses the mitochondrial apoptotic cascade during brain stem death. J Biol Chem, 2007. 282 : 4585 - 4600.

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宁夏医科大学学报
2013年 第9期

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