摘要
目的探讨力竭游泳后大鼠心肌基质金属蛋白酶(MMPs)活性的变化及其与运动性心肌重建和心功能异常的关系。方法建立一次性力竭游泳大鼠模型,通过胸超声心动图检测大鼠力竭游泳后即刻、3、6、12、24 h的心功能,包括主动脉血流峰值流速(PFVA)、主动脉血流速度积分(Viao)、左心室舒张末期直径(LVEDD)和左心室收缩末期直径(LVESD),并计算左室射血分数(LVEF)和左室短轴缩短率(LVFS);采用RT-PCR方法检测心肌MMP-1和MMP-9的mRNA含城,酶谱法分析心肌MMP-1和MMP-9活性;分析MMP-1和MMP-9活性变化与心功能指标之间的相关关系。结果力竭游泳后大鼠心肌MMP-1和MMP-9的mRNA含量水平显著高于对照组(P<0.01),MMP-1和MMP-9活性比对照组明显增加(P<0.01);与对照组比较,力竭游泳后大鼠PFVA、Viao、LVEF和LVFS显著减少(P<0.01);MMP-1和MMP-9 mRNA水平与PFVA、Viao、LVEF和LVFS呈负相关(P<0.05)。结论力竭游泳引起大鼠心肌MMPs的过度表达,并引起运动性心肌重建和心功能异常,研究提示,力竭游泳后大鼠心肌MMPs的过度表达可能与运动性心肌重建和心功能异常有关。
Objective To investigate the changes of myocardial matrix metalloproteinases(MMP)activities in rats after exhausted exercise and their relationship with exercise-induced cardidc remodeling and cardiac function.Methods The cardiac function indexes of rats such as peak flow velocity of aorta(PFVA),flow velocity integral of aorta(Viao),left ventricular cnd-diadtolic internal diameter(LVEDD),and left ventricular end-systolic internal diameter(LVESD) were determined by echocardiography at 0,3,6,12,24 hours after exhausted exercise.The left ventricular ejection fraction(LVEF) and left ventricular fractional shortening(LVFS) were calculated.MMP-1 and MMP-9 mRNA contains were measured with RT-PCR method,while MMP-1 and MMP-9activities were measured with SDS-PAGE type substrate gels embedded with type gelatin.Their relationship with cardiac function was analyzed.Results In exhausted exercise rats,both MMP-1 and MMP-9 mRNA contains and activities were increased significantly compared with those in controls(P <0.01),and the PFVA,Viao,LVEF and LVFS were reduced significantly compared with those in controls(P <0.01).Both MMP-1 and MMP-9 mRNA contains and activities were negatively correlated with PFVA,Viao,LVEF and LVFS(P <0.01).Conclusion The exerciseinduced cardiac remodeling and cardiac dysfunction induced by exhausted exercise may relate with over expression of MMP-1 and MMP-9.
出处
《兰州大学学报(医学版)》
CAS
2013年第4期7-12,共6页
Journal of Lanzhou University(Medical Sciences)
关键词
力竭游泳
心肌
基质金属蛋白酶
心脏收缩功能
exhausted exercise
myocardium
myocardial matrix metalloproteinases
cardiac function